Following isolation of CD4+ T cells, 5 million cells were plated onto each well of a 12-well non-treated TC dish that was coated overnight with 2 mug/mL of anti-CD3 antibody (Ultra-Leaf Purified anti-human CD3 Clone: OKT3, BioLegend, Cat#317326) and washed with 1XPBS, in the presence of RPMI-1640 complete medium containing 2 mug/mL of anti-CD28 antibody (Fitzgerald, Batch#0979) and 10 ng/mL IL-2 (Sigma-Aldrich, Cat#I7908-10KU).