6-10x106 PBMCs were rested in 15cm non-TC-treated plates (VWR) for 2 h. After 2 h, non-and semi-adherent cells were collected and cultured in complete RPMI medium supplemented with 50ng/ml GM-CSF and 100ng/ml IL-4 (Peprotech) for 6 days, replacing 2/3 media with complete RPMI+GM-CSF+IL-4 every other day to prepare monocyte-derive dendritic cells (MoDC).